University of Colorado Denver

LUTEAL PHASE LUPRON PROTOCOL

INSTRUCTIONS FOR USE OF INJECTABLE/ORAL MEDICATIONS
DURING THE IVF CYCLE

LUPRON (leuprolide acetate): If your first baseline ultrasound shows that your ovaries are free of large cysts, you will begin Lupron injections to suppress your natural ovulation cycle. Use a 50-unit insulin syringe to draw up your daily dose of 10 units. Administer in thighs or abdomen subcutaneously (sub-q). You will decrease the dose to 5 units daily when you begin your stimulation agent. GIVE THIS INJECTION IN THE EVENING within 2 hours of previous dose. (The use of generic Lupron is acceptable.)

STIMULATION AGENTS (Follistim, Gonal F, Fertinex, Pergonal, Repronex): After your second baseline ultrasound shows your ovaries to be free of large cysts and your blood estradiol is adequately low, you will be instructed to begin injections to stimulate your ovaries. Each time you have monitoring with ultrasound and/or blood work we will call you in the afternoon to review your dose until the next time we see you. You may be asked to take a single agent or a combination of agents. Your starting dose will be _____ ampules/vials (Follistim, Bravelle, Gonal F, Fertinex) daily and _____ ampules/vials (Repronex, Pergonal). Using a 3 cc syringe and a large (18-21) gauge 1½-inch needle, mix the ampules/vials of 75 IU powder in just 1 ml (1 cc) of sterile water (diluent). Do this by mixing the 1 ml water in the first ampule of powder and drawing it back into the syringe, then placing that mixture in the second ampule and drawing it back into the syringe, and so on until all _____ ampules of powder are in solution in the 1 ml water.

    For subcutaneous Follistim, Bravelle, Gonal F, or Repronex:Replace the large needle with a small (25-30) gauge ½-inch needle and inject subcutaneously (sub-q) in thighs or abdomen at a 90º angle. GIVE THIS INJECTION IN THE EVENING within one hour of previous dose.

    For intramuscular Pergonal: Replace the large needle with a small (25-27) gauge 1½-inch needle and inject in upper outer quadrant of buttock intramuscularly (IM) at a 90º angle. GIVE THIS INJECTION IN THE EVENING within 1 hour of previous dose.

HCG (chorionic gonadotropin, Profasi, Pregnyl, Novarel): After approximately 10 days of stimulation medication, when follicle sizes and estradiol levels are appropriate, you will be instructed to take no more Lupron or stimulation medication and to inject hCG at a specific time in the evening. You will be instructed on the day you are ready for hCG to take either 10,000 units or 5,000 units of hCG.

  • For 10,000 units: Using a 3 cc (ml) syringe and a large (18-21) gauge 1½-inch needle, draw up just 1 cc (ml) of sterile water (diluent). Inject the water into the 10,000 units hCG powder and withdraw the entire amount into the syringe. Replace the large needle with a small (25-27) gauge 1½-inch needle. After pulling back slightly on the plunger to check for blood, inject the medication in the upper outer quadrant of the buttock intramuscularly (IM) at a 90º angle.

  • If your dose is 5,000 units: Inject 2 cc (ml) of water into 10,000 units of hCG powder and withdraw just 1 cc (ml). Replace the large needle with a small (25-27) gauge 1½-inch needle. After pulling back slightly on the plunger to check for blood, inject the medication in the upper outer quadrant of the buttock intramuscularly (IM) at a 90º angle.

If blood is seen in the syringe after pulling back the plunger, remove the needle, replace it with a sterile 1½-inch needle, and inject at another IM site.

PROGESTERONE-IN-OIL: Progesterone is used to supplement your ovarian production of progesterone to ensure adequate support of an early pregnancy. Using a 3 cc syringe and a large (18-21) gauge 1½-inch needle, draw up 1 ml (cc), which is 50 mg. Replace the large needle with a small (22-25) gauge 1½-inch needle and inject intramuscularly (IM) in the upper outer quadrant of the buttock after pulling back on the plunger to check for blood. If blood is seen in the syringe after pulling back the plunger, remove the needle, replace it with a sterile 1½-inch needle, and inject at another IM site. GIVE THIS INJECTION IN THE EVENING within 1 hour of previous dose. Use of daily heating pad or hot moist towels 15 minutes daily on injection sites, and/or massage of the sites, helps absorption of the medication and helps to prevent soreness.

ABBREVIATIONS: Ml = cc
Sub-q = SQ = subcutaneously
IM = intramuscular
10 units = .1 cc
5 units = .05 cc
ampule = all glass container scored to facilitate breaking off the top
vial = container with a rubber top through which a sterile needle is inserted to mix and withdraw meds

MEDICATIONS BY MOUTH

ORAL CONTRACEPTIVE PILLS (OCP's, birth control pills, BCP's): https://pharma-doctor.com/norethindrone.html OCP's are used to suppress your ovulation and induce a resting state in your ovaries. Begin with the onset of your period when you are ready to proceed with your IVF cycle, take one active pill every day without interruption. DO NOT take the inactive spacer pills of which there are 7 of a different color in 28-day packs only. Discard the inactive pills as soon as you open the 28-day pack. You will continue daily pills through the first 5 days you take Lupron.

PRENATAL VITAMINS CONTAINING 400-1000 mcg (.4 to 1 mg) FOLIC ACID (folate): Take one daily when trying to conceive and throughout pregnancy.

LOW DOSE ASPIRIN 81 mg TABS: Aspirin thins your blood, which is expected to improve blood flow to ovaries and uterine lining. Begin taking one daily at or before the time you begin leuprolide. You will stop for a few days before and after the egg retrieval to prevent increased bleeding with the procedure.

DOXYCYCLINE: Doxycycline is an antibiotic given to prevent infection in your ovaries as a result of egg retrieval. If you are not allergic to tetracycline family drugs, begin one capsule twice daily the evening of egg retrieval. Always take doxycycline with food in your stomach and with lots of water to avoid irritation to your stomach and bowel. Doxycycline can cause hypersensitivity to the sun, so protect your skin with sunscreen, hats, and long sleeves when exposed to direct sun.

MEDROL: Medrol is a steroid which is given to women whose embryos undergo assisted hatching. The purpose of medrol is to decrease the risk of rejection and invasion of embryos whose outer shell has been thinned. You will be instructed to take 8 mg twice daily beginning the day following egg retrieval and continuing for 4 days.

University of Colorado Denver
Advanced Reproductive Medicine

Anschutz Medical Campus Office
Location: 1635 Aurora Court, Aurora, CO 80045
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